| 1. | It can provide the theoretic basis to study the stomatal movement by regulating the expression of transcription factors
为我们将来从调控转录因子的表达入手，人工干预气孔的开关运动提供理论上的依据。 |
| 2. | The genetic regulation of stomatal movement mainly depends on an efficient control system of gene expression , and guard cell - specific promoter is becoming one of the best choices
因此有必要对气孔的开关进行调控。气孔运动的分子调控离不开高效的基因开关系统，保卫细胞特异性表达的启动子近几年倍受关注。 |
| 3. | However there was little knowledge about the function of atdofl . 7 , especially to stomatal movement . we constructed plant expression vector driven by camv35s promoter and obtained tl transgenic tobaccos
为了深入理解调控气孔运动的分子机制，除了对保卫细胞特异性表达启动子进行研究外，还对与气孔运动相关的转录因子进行了研究。 |
| 4. | These include phototropism , light - driven chloroplast movements and stomatal movements . the phot i gene of arabidopsis encodes an autophosphosphorylating protein kinase that functions as a photoreceptor for phototropism in response to low - intensity bl . up to now
在高等植物中，蓝光调控多个重要生理反应过程，其中包括下胚轴的向光反应、叶绿体在光下的重新分布和气孔的开放等。 |
| 5. | The results represented upon observation of stomatal movement showed that the density of stoma in the epidermal of transgenic tobaccos was much lower than that of wild type and the rate of opening and closing of stoma increased because of strong expression oi atdofl . 7
我们利用rt - pcr的方法，从拟南芥中克隆了与在气孔中表达较多的stdof1基因的同源基因atdof1 . 7 ，构建了该基因的超表达载体，获得了t1代转基因烟草苗。 |
| 6. | However , gus activity in leaves and epidermal strips increased with the prolongation of treatment time . in order to further understand the regulatory mechanism of stomatal movement , we studied one of transcription factors that might be related to stomatal movement . using rt - pcr we isolated atdofl . 7 from arabidopsis , which had a higher homology with stdofl whose expression was very high in guard cells
Gus活性荧光定量分析表明， sigp1启动子驱动的gus活性在干旱、 aba 、高盐和冷等逆境处理下，在根的表达很微弱，且随着处理时间的延长变化不大，但在叶片和表皮中的gus活性都有增加的趋势。 |
| 7. | Most interestingly , on the contrary to aba , the dcf fluorescence intensity of guard cells treated by 100 umol l - 1 salicylic acid ( sa ) was not down - regulated by pd98059 , yet pd98059 did not regulate the stomatal movement being induced by light , dark or salicylic acid
但是， pd98059和sa共同处理， hzoz探针荧光强度升高; sa诱导dcf荧光强度升高后，以pd98059处理，对dcf荧光强度无影响;将pd98059注射进入其中的一个保卫细胞，以sa处理，结果显示两个细胞内的荧光强度一样。 |
| 8. | Cdpks play important roles in plant calcium signal transduction . there is increasing evidence that cdpks participate in c / n metabolism , transmembrane transport of ion and water , cytoskeletal regulation , stomatal movement regulation , growth and development regulation and biotic and abiotic stress responses in plants
Cdpks在植物钙信号转导中具有重要的作用，越来越多的证据表明，在植物碳氮代谢、离子和水分跨膜运输、细胞骨架调节、气孔运动调节、生长发育调节以及生物和非生物胁迫应答反应中均有cdpks的参与。 |
| 9. | This new method will bring significant developments in studying the principles of stomatal movement , and other quick movement in plants , c ) guard cells are incubated with ph dependent fluorescent chemical probe " bcecf am " and excited at 488nm , the fluorescent emission ratio method ( 520nm / 640nm ) is employed with laser scanning confocal microscopy , about 0 . 4 ph unit increase in guard cell vacuoles is observed during stomatal closure that is induced by aba
本发展为保卫细胞与其它小细胞液泡的进一步研究提供了新思路。 c )本工作通过激光共聚焦显微术配合ph荧光探针bcecfam的单激发( 488nm )双发射的荧光比值法( 525nm 640nm )观察到，用aba处理的表皮条上的开放态气孔在关闭过程中其保卫细胞液泡内ph有一约0 . 4单位的上升。 |
| 10. | Additionally , protein bands were found in the range of 62 kd in microsomes from oryza sativa and 45 kd from arabidopsis thaliana - , 2 ) the ipar - like protein was mainly localized in the vacuolar membrane ( tonoplast ) and plasma membrane in the leaf and root tip of arabidopsis thaliana for confirming the biological role of the ip3r - like protein , we investigated the effects of methyl viologen ( stimulating ip3 produce ) and heparin ( a competitive inhibitor of inositol 1 , 4 , 5 - triphophate in animal ) on stomatal movement by epidermal strip bioassay . when the epidermal strips of arabidopsis thaliana were treated with methyl viologen , it stimulates the stomatal closure
拟南芥表皮条用甲基紫精处理，可以引起气孔的关闭，当处理90分钟的时候，气孔的相对开度由100降到58 ，统计学分析气孔开度的变化有显著差异；甲基紫精引起气孔关闭的作用可以被肝素部分抑制，当甲基紫精和肝素同时处理90分钟的时候，气孔的相对开度由100降到92 ，统计学分析气孔开度的变化没有显著差异，说明气孔的相对开度基本上没有变化。 |
